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. 2023 Apr 12;616(7958):764–773. doi: 10.1038/s41586-023-05927-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 21 — a. Representative images of tdTomato+ striatal astrocytes in SAPAP3 KO mice injected with Astro GFP (left) or Astro SAPAP3 (right). Bar graph shows the measurements of astrocyte territory area. n = 20 cells from 4 mice per group. The mean and SEM are shown (Astro GFP v Astro SAPAP3, two-tailed unpaired t-test with Welch correction, P = 0.001). b. Representative images of PLA puncta for SAPAP3 and Ezrin in SAPAP3 KO mice injected with Astro GFP or Astro SAPAP3. Images show SAPAP3-Ezr puncta within a tdTomato+ astrocyte. Bar graph denotes the integrated density in arbitrary units (a.u.) of the PLA signal within the astrocyte ROI. n = 12 tdTomato+ astrocytes from 4 mice per group. The mean and SEM are shown (Astro GFP v Astro SAPAP3, two-tailed unpaired t-test with Welch correction, P = 0.002). c. Representative images of PLA puncta for SAPAP3 and Glt-1 in SAPAP3 KO mice injected with Astro GFP or Astro SAPAP3. Images show SAPAP3-Glt-1 puncta within a tdTomato+ astrocyte. Bar graph denotes the integrated density in arbitrary units (a.u.) of the PLA signal within the astrocyte ROI. n = 12 tdTomato+ astrocytes from 4 mice per group. The mean and SEM are shown (Astro GFP v Astro SAPAP3, two-tailed unpaired t-test with Welch correction, P < 0.001). d. Representative images of tdTomato+ striatal astrocytes in SAPAP3 KO mice injected with Neuro GFP (left) or Neuro SAPAP3 (right). Bar graph shows the measurements of astrocyte territory area. n = 20 cells from 4 mice per group. The mean and SEM are shown (Neuro GFP v Neuro SAPAP3, two-tailed unpaired t-test with Welch correction). e. Representative images of PLA puncta for SAPAP3 and Ezrin in SAPAP3 KO mice injected with Neuro GFP or Neuro SAPAP3. Images show SAPAP3-Ezr puncta within a tdTomato+ astrocyte. Bar graph denotes the integrated density in arbitrary units (a.u.) of the PLA signal within the astrocyte ROI. n = 12 tdTomato+ astrocytes from 4 mice per group. The mean and SEM are shown (Neuro GFP v Neuro SAPAP3, two-tailed Mann-Whitney test, P = 0.59). f. Representative images of PLA puncta for SAPAP3 and Glt-1 in SAPAP3 KO mice injected with Neuro GFP or Neuro SAPAP3. Images show SAPAP3-Glt-1 puncta within a tdTomato+ astrocyte. Bar graph denotes the integrated density in arbitrary units (a.u.) of the PLA signal within the astrocyte ROI. n = 12 tdTomato+ astrocytes from 4 mice per group. The mean and SEM are shown (Neuro GFP v Neuro SAPAP3, two-tailed Mann-Whitney test, P = 0.98).