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. 2023 Apr 26;13:6821. doi: 10.1038/s41598-023-32293-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Our proposed pipeline, Seurat, and ScanPY can analyze and identify all major cell types in the human heart cell atlas data. 2D plots visualize the cell clustering and cell-type in (A) AI-based Autoencoder (UMAP plot), (B) Seurat (UMAP plot), (C) ScanPY (ScanPY coordinate plot). V-CM (red): ventricular cardiomyocyte. A-CM (ligh red): atrial cardiomyocyte. EC (blue): endothelial cell. PC (cyan): pericyte. SMC (pink): smooth muscle cell. FB (green): fibroblast. Mono (yellow): monocyte and macrophage. Lympho (violet): lymphocyte, including T cells and natural killer cells. GLI (grey): neuronal (glial) cell.