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. 2023 Apr 26;13:6857. doi: 10.1038/s41598-023-33941-2

Figure 4.

Figure 4

Increasing cell processing throughput for clinical-scale volumes. (A) Photograph of a 2 mm and (B) 10 mm electroporation flow cell. Red arrows highlight the channel width. (C) Plot of GFP expression and viability values from Jurkat cells transfected with mRNA encoding GFP in either the 2- or 10-mm channels (n = 3). (D) Plot of GFP expression and viability values from Jurkat cells transfected with mRNA encoding GFP in the 2-mm channel at varying cell concentrations in the electroporation buffer (n = 3). (E) Plot of GFP expression and viability values from Jurkat cells transfected with mRNA encoding GFP in the 10-mm channel at varying flow rates and waveform frequencies (n = 3). (F) Plot of GFP expression and viability over time from an experiment that transfected ~ 240 million cells over 56 s (n = 1). Data shown as mean ± standard deviation (C–E). Some error bars are too small to be visible (C–E).