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. 2023 Feb 24;298(3):537–548. doi: 10.1007/s00438-023-01994-7

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Deletion of MMJJ_06980, a predicted PstI restriction enzyme, increases the transformation efficiency of transformations with integrative plasmids: Transformation efficiencies of KC13 compared to ΔMMJJ_06980 with either pCRUptNeoΔflaK or pLW40neo. Data are averages from three independent experiments, and error bars represent one standard deviation around the mean. Statistics were performed using a two-tailed, equal variance T test. n.s, not significant (p. 0.05); * p < 0.05; ** p < 0.01; *** p < 0.001