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. 2023 Feb 24;298(3):537–548. doi: 10.1007/s00438-023-01994-7

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — MMJJ_13030 is essential to transformation in M. maripaludis and its homolog is essential to transformation in M. thermophilus DSM 2373. A Transformation efficiencies with and without MMJJ_13030 of M. maripaludis JJ. EV denotes KC13 + pLW40neo (empty vector), and Comp. refers to ΔMMJJ_13030 + pLW40neo-MMJJ_13030. KC13 and KC13 + EV paired with respective mutants were transformed on the same day with the same transforming DNA (tDNA). Data are averages from three independent experiments, and error bars represent one standard deviation around the mean. B Transformation efficiencies of M. thermophilus DSM 2373 and a ∆SAMN04488571_104187 strain transformed on same day with same transforming DNA. Data are averages from three independent experiments, and error bars represent one standard deviation around the mean