Table 1.

Insertions identified across both transposon screens

Locus tag	Annotation	Genomic location (nucleotide position) of insertions identified with pLW40neo as substrate	Genomic location (nucleotide position) of insertions identified with pCRuptNeoΔflaK as substrate
MMJJ_01810	PC4 domain-containing protein	172,367	–
MMJJ_03840	Class III signal peptide	365,933	–
MMJJ_07800/ MMJJ_07810	CBS domain-containing protein / Pheromone shutdown-related protein TraB	722,257 (intergenic)	–
MMJJ_08190	epdI	754,017; 754,141; 754,574; 754,702; 754,810; 755,436	754,525; 754,557
MMJJ_08200	epdH	755,754; 755,796; 755,904; 755,921	–
MMJJ_08210	DUF2341 domain-containing protein	756,173; 756,194; 756,445; 756,977; 757,563; 757,807	756,103; 756,104; 756,456
MMJJ_08230	Uncharacterized protein	758,274	758,229; 758,271; 753,363
MMJJ_08240	epdC	–	758,553; 758,560
MMJJ_08260	Uncharacterized protein	760,097	–
MMJJ_08270	epdF	760,641; 760,998	761,067
MMJJ_08280	epdA	761,556	–
MMJJ_08290	eppA	762,641	762,811
MMJJ_10500	Uncharacterized protein	961,509	–
MMJJ_10560	epdL	967,156; 968,330	–
MMJJ_10570	epdK	968,871	968,941; 969,459
MMJJ_10580	epdJ	970,403; 970,619	–
MMJJ_11080	MinD-like ATPase involved in chromosome partitioning or flagellar assembly	1,026,741; 1,027,115; 1,027,298	1,027,299
MMJJ_11090	Uncharacterized protein	1,027,598; 1,028,171; 1,028,366; 1,028,570; 1,028,813; 1,028,953; 1,028,965	1,027,582; 1,028,952; 1,029,061
MMJJ_13020	OB-fold nucleic acid binding domain protein	1,193,088	1,193,041; 1,193,089
MMJJ_13030	Tripartite tricarboxylate transporter TctA family protein	–	1,194,079
MMJJ_13210	Peptidase family M50	1,210,579	–
MMJJ_14410	Putative succinyl-diaminopimelate desuccinylase	1,306,596	1,306,596; 1,306,683
MMJJ_16440	Thermonuclease	–	1,499,668
MMJJ_17200	Archaeosortase family protein ArtE	–	1,571,918
MMJJ_17710	Uncharacterized protein	1,619,404; 1,619,626	–