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Electrophoresis of PCR-RFLP for PCR product (273bp) with a FokI restriction enzyme, 3% agarose, 75 V, 20 mA for 2 h.
(LaneM: DNA marker [100 bp], Lane: 2, 3, 4, 7, 8, 10, 11 homozygote type FF genotype, Lane: 1,9 heterozygote mutant type Ff genotype, Lane: 5,6 homozygote mutant type ff genotype)
