Figure 3.

Pure culture techniques developed by Emil Christian Hansen and Paul Lindner. Two different methods for the generation of pure monocultures of yeast were developed by Hansen and Lindner. Hansen’s method was a fractionated single-colony streak on solid medium whereas Lindner’s involved single droplet isolation under the microscope. In Lindner’s method, mini droplets of a liquid sample (e.g. wort, green beer, and beer) were placed onto scratches of a glass slide and viewed using a microscope. If there were single cells in the field, they could be transferred into sterile wort by using sterile blotting paper. Both methods were adopted by breweries and used to develop pure cultures of S. pastorianus from Stellhefen mixtures of brewing yeasts.