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Binarization, morphological operations, contouring, and feature extraction at imaging time point for image sets-1 (0.5 agarose), 2 (0.8 agarose), and 3 (1.5 agarose) at the first, second, and third rows, respectively. To illustrate that these calculations were made for all 27 imaging time points for 3 image sets, area, perimeter, and radius values for imaging time point-5 were given here.
