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. 2022 Dec 19;12(7):8289–8305. doi: 10.1002/cam4.5527

FIGURE 3.

FIGURE 3

The overexpression of circSWT1 promotes EMT in NSCLC. (A) A549 cells overexpressing circSWT1 displayed a needle‐like morphology, but H1299 cells with circSWT1 knockdown presented no significant differences. (B) Relative RNA level of E‐cadherin, N‐cadherin, and vimentin in A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells was measured by qRT‐PCR. (C) Relative protein levels of E‐cadherin, N‐cadherin and imentin in A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells were assessed by western blotting. (D) Immunofluorescence staining of E‐cadherin and N‐cadherin in A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells. (E) The paraffin sections were split into two groups based on circSWT1 level, and IHC was performed to assess the levels of E‐cadherin and N‐cadherin in the two groups. (F) The correlation between circSWT1 and E‐cadherin and N‐cadherin was analyzed. CircSWT1 level was measured by qRT‐PCR, and the level of E‐cadherin and N‐cadherin was measured by IHC. The data are presented as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001.