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. 2022 Dec 19;12(7):8289–8305. doi: 10.1002/cam4.5527

FIGURE 5.

FIGURE 5

The effect of circSWT1 on the progression of tumors and EMT is reversed by knocking out SNAIL in NSCLC. (A) Relative protein levels of E‐cadherin, N‐cadherin, and vimentin were assessed by Western blotting of A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells with SNAIL knockout. (B) qRT‐PCR was used to determine the relative RNA levels of E‐cadherin, N‐cadherin, and vimentin in A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells with SNAIL knockout. (C) The deletion of SNAIL in A549‐circSWT1 and H1299‐shcircSWT1 cells did not result in a spindle‐like shape. (D) Immunofluorescence staining of E‐cadherin and N‐cadherin in A549 (A549‐control and A549‐circSWT1) and H1299 (H1299‐control and H1299‐shcircSWT1) cells with SNAIL knockout. (E) Matrigel Transwell assays were performed to assess the invasion of A549‐circSWT1 and H1299‐shcircSWT1 cells with SNAIL knockout. (F) Colony formation assays and (G) CCK‐8 assays were performed to evaluate the viability of A549‐circSWT1 and H1299‐shcircSWT1 cells with SNAIL knockout. (H) The migration of A549‐circSWT1 and H1299‐shcircSWT1 cells with SNAIL knockout was not significantly different from that of the control group. The data are presented as the mean ± SD of three independent experiments. ***p < 0.001, ns: not significant.