Skip to main content
. 2023 Jan 23;12(7):8510–8525. doi: 10.1002/cam4.5601

FIGURE 5.

FIGURE 5

POSTN Iso5 cooperatively promotes invasion with Iso3 which is conventional isoform. (A) HOC621 cells were engineered to overexpressing GFP, POSTN Iso3 and/or Iso5 by transfection with lentiviral gene transfer. The overexpression of GFP was used as a control. Ectopic expression of POSTN Iso3 and Iso5 was quantified by qRT‐PCR. In each group, data are presented as the mean ± SD of triplicates. ****p < 0.001, ***p < 0.005, **p < 0.01. (B) Ectopic expression of POSTN Iso3 and Iso5 were detected by RT‐PCR using primer set F2/R2. GAPDH was used as a loading control. (C) Ectopic expression of Periostin Iso3 and Iso5 with monensin treatment was examined by immunoblotting with antibodies detecting N‐terminus and exon 21 of POSTN. anti‐Ex21 periostin antibody can detect POSTN Iso5 but not Iso3 lacking Exon 21. β‐Actin was used as a control. (D) The invasion ability of GFP‐, POSTN Iso3‐, Iso5, and Iso3/5‐overexpressing HOC621 cells were examined by in vitro invasion assay. Representative images were shown. Graph shows the number of invaded cells as mean ± SEM (n = 3). ****p < 0.001, ***p < 0.005, **p < 0.01.