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. 2023 Jan 23;12(7):8510–8525. doi: 10.1002/cam4.5601

FIGURE 6.

FIGURE 6

Knockdown of POSTN Iso5 attenuates invasion ability in HNSCC cell line having EMT feature. (A) The structure of POSTN Iso3 and Iso5 are shown. Moreover, siRNA targeting POSTN Iso5 (siEx21) or total POSTN are shown. (B) POSTN mRNA expression in Iso5‐overexpressing HOC621 cells were determined by qRT‐PCR. In each group, data are presented as the mean ± SD of triplicates. ****p < 0.001 (C) POSTN mRNA expression in negative control siRNA (siNC), total POSTN siRNA (siPOSTN), and siRNA targeting POSTN Iso5 (siEx21)‐transfected HOC719‐NE cells were determined by qRT‐PCR. In each group, data are presented as the mean ± SD of triplicates. ****p < 0.001 (D) POSTN isoforms were detected by RT‐PCR using primer set F2/R2 in siNC, siPOSTN, and siEx21‐transfected HOC719‐NE cells. GAPDH was used as a control. (E) The invasion ability of siNC and siEx21‐transfected HOC719‐NE cells were determined by in vitro invasion assay. Representative images were shown. Graph shows the number of invaded cells as mean ± SEM (n = 3). ****p < 0.001.