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. 2023 Jan 23;12(7):8510–8525. doi: 10.1002/cam4.5601

FIGURE 8.

FIGURE 8

Induction of p‐EMT genes by POSTN Iso3 and/or Iso5. (A) HOC621 cells were engineered to overexpressing POSTN Iso3 and/or Iso5 by transfection with lentiviral vector. The overexpression of GFP was used as a control. Expression of p‐EMT genes was evaluated by qPCR in EV‐, POSTN Iso3‐, Iso5, and Iso3/5‐transfected HOC621 cells. The graphs show the results as mean ± S.D. of three independent experiments. *p < 0.05 (compared to EV). (B) Expression of p‐EMT genes was evaluated by qPCR in negative control siRNA (siNC), siRNA targeting POSTN Iso3 and 5 (siPOSTN), and siRNA targeting POSTN Iso5 (siEx21)‐transfected HOC719‐NE cells with both POSTN Iso3 and Iso5 expression. The graphs show the results as mean ± S.D. of three independent experiments. *p < 0.05 (compared to siNC).