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Effect of reaction mixture purification on the Signal‐to‐Noise ratio (SNR). (A) Results of somatic mutation and MSI status detection by MASE‐CE without purification (top panel) or with purification (bottom panel). (B) Different SNR with different amount of enzyme for purification. Signal‐to‐Noise ratio (SNR) equal to Expected peak/Extraneous peaks. KRAS‐G12C, KRAS c.34G > T; KRAS‐A146T, KRAS c.436G > A; NRAS‐G12C, NRAS c.34G > T; BRAF‐V600E, BRAF c.1799 T > A.
