KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| MDHC (H-6) | Santa Cruz Biotechnology | Cat# sc-166879, RRID:AB_10609257 |
| GPD1 Polyclonal antibody | proteintech | Cat# 27943-1-AP, RRID:AB_2881016 |
| GPD1L Polyclonal antibody | proteintech | Cat# 17263-1-AP, RRID:AB_2112359 |
| β-tubulin antibody (HRP conjugated) | Cell signaling | Cat# 5346 RRID:AB_1950376 |
| Goat anti-rabbit secondary antibodies (HRP conjugated) | LI-COR | Cat# 926-80011 RRID:AB_2721264 |
| Anti-mouse secondary antibody (m-IgGκ BP-HRP) | Santa Cruz Biotechnology | Cat# sc-516102, RRID:AB_2687626 |
| Rabbit anti-Ras (G12V), monoclonal | Cell Signaling | Cat.#: 14412, RRID:AB_2714031 |
| Chemicals, peptides, and recombinant proteins | ||
| Sodium 2-oxobutyrate (AKB) | Sigma | K0875 |
| Sodium pyruvate | Sigma | P5280 |
| Critical commercial assays | ||
| BCA protein assay | Thermo Fisher | Cat.#: 23225 |
| SuperScript III First-Strand Synthesis SuperMix | Invitrogen | Cat#18080400 |
| Maxima SYBR Green/ROX qPCR Master Mix (2X) | Thermo Scientific | K0221 |
| NAD/NADH Quantitation Colorimetric Kit | Biovision | Cat#K337 |
| Deposited Data | ||
| Raw image data | This manuscript | DOI: 10.17632/btc9bbcch8.1 |
| Original code | This manuscript | https://github.com/e-stan/NADH_KFP |
| Experimental models: Cell lines | ||
| 3T3 | American Type Culture Collection | RRID:CVCL_0123 |
| PC-3 | American Type Culture Collection | RRID:CVCL_0035 |
| DITNC1 | American Type Culture Collection | |
| BT549 | American Type Culture Collection | RRID:CVCL_1092 |
| HCT 116-Luc2 | American Type Culture Collection | Cat# CCL-247-LUC2, RRID:CVCL_VU38 |
| Experimental Models: Organisms/Strains | ||
| Mouse: 43-56 days old female nude mice | Charles River Laboratories | 490CRATHHO |
| Oligonucleotides | ||
| Negative Control DsiRNA | IDT | Cat.#: 51-01-14-04 |
| DsiRNA: GPD1L (human) | IDT TriFECTa DsiRNA Kit | hs.Ri.GPD1L.13 |
| DsiRNA: MDH1(human) | IDT TriFECTa DsiRNA Kit | hs.Ri.MDH1.13 |
| DsiRNA: GPD1L(mouse) | IDT TriFECTa DsiRNA Kit | mm.Ri.Gpd1l.13 |
| DsiRNA: MDH1(mouse) | IDT TriFECTa DsiRNA Kit | mm.Ri.Mdh1.13 |
| MDH1 primer 1: Sequence: GGT TAC ACC GAG TTT AAG AGC A | IDT PrimeTime® qPCR Primers | Mm.PT.58.5174962 |
| MDH1 primer 2: Sequence: CCA ATA CGA ACT GCC TGA CA | IDT PrimeTime® qPCR Primers | Mm.PT.58.5174962 |
| GPD1 primer 1: Sequence: TGT CAC CGA AGC CAA GC | IDT PrimeTime® qPCR Primers | Mm.PT.56a.32463908 |
| GPD1 primer 2: Sequence: ACC CAA CTT TCG CAT CAC T | IDT PrimeTime® qPCR Primers | Mm.PT.56a.32463908 |
| GPD1L primer 1: Sequence: TCT TGT GGA TGA ACT GGT GAG | IDT PrimeTime® qPCR Primers | Mm.PT.58.7971254 |
| GPD1L primer 2: Sequence: AAC GTG AAA TAT CTC CCA GGA C | IDT PrimeTime® qPCR Primers | Mm.PT.58.7971254 |
| LDHA primer 1: Sequence: CCC TTG AGT TTG TCT TCC ATG A | IDT PrimeTime® qPCR Primers | Mm.PT.58.29860774 |
| LDHA primer 2: Sequence: CTC CCC AGA ACA AGA TTA CAG T | IDT PrimeTime® qPCR Primers | Mm.PT.58.29860774 |
| B2M primer 1: Sequence: GGG TGG AAC TGT GTT ACG TAG | IDT PrimeTime® qPCR Primers | Mm.PT.39a.22214835 |
| B2M primer 2: Sequence: TGG TCT TTC TGG TGC TTG TC | IDT PrimeTime® qPCR Primers | Mm.PT.39a.22214835 |
| Recombinant DNA | ||
| Mdh1_OMu15660D_pcDNA3.1+/C-(K)-DYK | Genscript | Clone ID:OMu15660D ORF Clones (Accession No.):NM_008618.3 (ORF Sequence),1002 bp Vector:pcDNA3.1+/C-(K)-DYK |
| Gpd1_OMu18825D_pcDNA3.1+/C-(K)-DYK | Genscript | Clone ID:OMu18825D ORF Clones (Accession No.):NM_010271.3 (ORF Sequence),1047 bp Vector:pcDNA3.1+/C-(K)-DYK |
| MDH1_OHu13813D_pcDNA3.1+/ C-(K)-DYK | Genscript | Clone ID:OHu13813D ORF Clones (Accession No.):NM_005917.4 (ORF Sequence),1002 bp Vector:pcDNA3.1+/C-(K)-DYK |
| GPD1_OHu20325D_pcDNA3.1+/C-(K)-DYK | Genscript | Clone ID:OHu20325D ORF Clones (Accession No.):NM_005276.4 (ORF Sequence),1047 bp Vector:pcDNA3.1+/C-(K)-DYK |
| Human MDH1res | Genscript | codon-optimized cDNA of human MDH1 in pcDNA3.1+/C-(K)-DYK (siRNA-resistant) |
| Mouse MDH1res | Genscript | codon-optimized cDNA of mouse MDH1 in pcDNA3.1+/C-(K)-DYK (siRNA-resistant) |
| Human GPD1Lres | Genscript | codon-optimized cDNA of human GPD1L in pcDNA3.1+/C-(K)-DYK (siRNA-resistant) |
| Mouse GPD1Lres | Genscript | codon-optimized cDNA of mouse GPD1L in pcDNA3.1+/C-(K)-DYK (siRNA-resistant) |
| GFP_pcDNA3.1+/C-(K)-DYK | Genscript | Vector name: pcDNA3.1+/C-(K)-DYK |
| pCMV-VSV-G | (Yao et al., 2019) | Addgene plasmid # 8454 |
| pCMV delta R8.2 | (Yao et al., 2019) | Addgene plasmid # 12263 |
| pLVX-HRasV12- hygromycin | (Yao et al., 2019) | |
| Purified lentiviral particles for human MDH1 (NM_005917.3) in pReceiver-Lv105 with CMV promoter, puromycin | GeneCopoeia | LPP-C0441-Lv105-050-S |
| Lentifect™ Purified Lentiviral Particles for Negative Control for pReceiver-Lv105 | GeneCopoeia | LP105-025 |
| Software and algorithms | ||
| Python (v3.7) | NA | www.python.org |
| NumPy (v1.19.5) | NA | www.numpy.org |
| Pandas (v1.0.1) | NA | www.pandas.pydata.org |
| SciPy (v1.4.1) | NA | www.scipy.org |
| Matplotlib (v3.1.3) | NA | www.matplotlib.org |
| Other | ||
| Lipofectamine™ RNAiMAX Transfection Reagent | Thermo Fisher | Cat.#: 13778075 |
| Lipofectamine™ 3000 Transfection Reagent | Thermo Fisher | Cat.#: L3000001 |
| Lipofectamine™ 2000 Transfection Reagent | Thermo Fisher | Cat.#: 11668019 |