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. Author manuscript; available in PMC: 2023 Apr 27.
Published in final edited form as: ACS Nano. 2021 Aug 6;15(10):15741–15753. doi: 10.1021/acsnano.0c09553

Figure 8.

Figure 8.

Efficacy studies in an EcoHIV-infected meth-treated animal model. C57BL/6 male mice were divided into 12 groups and were assigned to the following treatment groups: control virus + meth + saline (12 animals); control virus + saline (12 animals); control virus + meth + T-CoQ10-NP/T-(Asp)4-NP (13 animals); EcoHIV + meth + saline (11 animals); EcoHIV + meth + T-CoQ10-NP/T-(Asp)4-NP (10 animals); EcoHIV + meth + T-ART-NPs (12 animals); EcoHIV + meth + T-ART-NP + T-CoQ10-NP/T-(Asp)4-NP (16 animals); control virus + meth + T-CoQ10/(Asp)4-NPs (11 animals); control virus + T-ART-NPs (13 animals); control virus + T-ART-NPs + T-CoQ10/(Asp)4-NPs (12 animals), saline (3 animals), and EcoHIV + saline (3 animals). (A) Timeline illustrating infection of C57BL/6 male mice with either control virus (pBMN-I-GFP) or chimeric HIV-NDK (EcoHIV, 1 μg of p24), followed by one-week multiday exposure to meth (0.2 mg/kg at each injection for 5 days) and subsequent two-week treatment with T-ART-NPs (5 mg/kg with respect to the drug) and T-CoQ10/(Asp)4-NPs (20 mg/kg with respect to the drug). (B) Levels of EcoHIV p24 antigen in blood by ELISA and in the brain by RT-PCR. (C) Inflammatory markers IL-1β and TNF-α as measured by ELISA in blood plasma. (D) Neuroinflammation markers C3 and OLFM1 measured by RT-PCR in the brain. (E) ROS markers glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and glutathione peroxidase 7 (GPX7) were measured using RT-PCR in the brain.