Mice were treated as in Fig. 1. (A) TG and cholesterol levels were measured. (B) Adipose tissue/BW ratio was quantified. (C) Representative images of H&E staining of eWAT. (D) Distribution of adipocyte area of eWAT was analyzed. Around 1191–2294 adipocytes from 2 to 3 mice in each group were quantified. Data are expressed as means ± SEM (n = 4 to 8, A, B) and subjected to one-way ANOVA with Turkey post hoc test. #P < 0.05 vs. WT EtOH; ^P < 0.05 vs. LKO EtOH; &P < 0.05 vs. WT control; $P < 0.05 vs. LKO control. Original magnification, × 20. Abbreviations: BW, body weight; EtOH, ethanol; eWAT, epididymal white adipose tissue; H&E, hematoxylin and eosin; iBAT, interscapular brown adipose tissue; iWAT, inguinal white adipose tissue; KO, knockout; mTOR, mechanistic target of rapamycin; TG, triglyceride; WT, wild-type.