Fig. 4. Acute alcohol exposure leads to steatosis and liver injury and Rptor^AKO and Rptor^LAKO mice have exacerbated liver injury.

Rptor wild-type (Rptor^WT), liver-specific Rptor KO (Rptor^LKO), adipocyte-specific Rptor KO (Rptor^AKO) as well as liver- and adipocyte-specific Rptor KO (Rptor^LAKO) mice were generated by crossing floxed Rptor mice with albumin Cre or adiponectin Cre mice. The mice were treated with ethanol 7 g/kg or equal amount of water by oral gavage for 6 h. (A) Representative images of H&E staining of the livers. (B) Hepatic TG and cholesterol were quantified. (C) Liver/body weight ratio was quantified. (D) Serum ALT activities were measured. Data are expressed as means ± SEM (n = 4 to 7) and subjected to one-way ANOVA with Turkey post hoc test. *P < 0.05 EtOH vs. control; #P < 0.05 vs. WT EtOH; ^P < 0.05 vs. LKO EtOH; &P < 0.05 vs. WT control; $P < 0.05 vs. LKO control. Original magnification, × 20. Abbreviations: ALT, alanine aminotransferase; EtOH, ethanol; H&E, hematoxylin and eosin; KO, knockout; Rptor, regulatory-associated protein of mTOR; TG, triglyceride; WT, wild-type.