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. 2023 Mar 22;97(4):e01907-22. doi: 10.1128/jvi.01907-22

FIG 6.

FIG 6

Treatment with IFN-β, but not IFN-λ1, inhibits MCPyV postentry activities. (A) MCPyV-infected HDFs were either untreated or treated with 1,000 U/mL IFN-β, 1,000 U/mL IFN-λ1, or both for 30 min before FBS was added to the medium on day 2 postinfection. Cells were harvested for DNA extraction on day 5 postinfection. MCPyV genomic DNA levels were determined by qPCR quantification and normalized to the GAPDH genomic DNA levels. The value for one of the MCPyV-infected HDF groups with no IFN treatment was set as 1. (B) HDFs infected with MCPyV were either untreated or treated with 100 U/mL IFN-β at the time points indicated. Cells were harvested for DNA extraction on day 5 postinfection. MCPyV genomic DNA levels were determined by qPCR quantification and normalized to the GAPDH genomic DNA levels. The value for one of the MCPyV-infected HDF samples with no IFN treatment was set as 1. Error bars represent the standard deviations from three independent experiments. ***, P < 0.001; ns, not significant.