FIG 2.

Relative levels of MEP pathway intermediates and downstream metabolites GPP and FPP. DXP and MEcDP are the most abundant MEP pathway metabolites in wild-type Z. mobilis. Intracellular metabolite concentrations were measured using an isotope ratio-based approach (see Materials and Methods). Data represent averages of six biological replicates. See Table 1 for the absolute metabolite concentrations of these metabolites. DXP, 1-deoxy-d-xylulose 5-phosphate; MEP, 2-C-methyl-d-erythritol 4-phosphate; CDP-ME, 4-diphosphocytidyl-2-C-methyl-d-erythritol; MEcDP, 2-C-methyl-d-erythritol 2,4-cyclodiphosphate; HMBDP, 4-hydroxy-3-methylbut-2-enyl diphosphate; IDP, isopentenyl diphosphate; DMADP, dimethylallyl diphosphate; GPP, geranyl pyrophosphate; FPP, farnesyl pyrophosphate.