TABLE 2.
Isoprene production in Z. mobilis strains overexpressing isoprene synthase
| Strain name | Overexpressing gene(s) | Isoprene production (nmolisoprene mmolglucose−1)a |
|---|---|---|
| ZM4_GFP | GFP | 5.6 ± 0.5 (n = 4) |
| ZM4_IspS | IspS | 5.8 ± 2.9 (n = 3) |
| ZM4_DXS2_IspS | DXS2, IspS | 33.3 ± 8.6b (n = 5) |
| ZM4_DXS2_IspS_IDI | DXS2, IspS, IDI | 16.3 ± 3.7 (n = 8) |
| ZM4_DXS2_IspG_IspH_IspS | DXS2, IspG, IspH, IspS | 18.1 ± 4.0 (n = 5) |
| ZM4_DXS2_IspG_IspH_IspS_IDI | DXS2, IspG, IspH, IspS, IDI | 20.1 ± 5.9b (n = 5) |
a
Isoprene production represents the average of at least three biological replicates per strain ± standard error.
b
Statistically significant difference in isoprene production compared to ZM4_GFP (P < 0.05).