FIG 2.

NCI05 binds to the coil/helical conformation of V2. (A) Crystal structure of NCI05 in complex with V2 peptide V2c at 2.35 Å resolution. (Left) the refined structure of antibody and V2 peptide complex with NCI05 heavy chain (orange), light chain (gray), and V2 peptide (green). CDRs are colored as follows: H1, magenta; H2, olive; H3, orange; L1, light blue; L2, navy blue; and L3, purple. (Center) Two 90° rotations of the surface representation show that the V2c peptide binds to the groove formed by CDRs H2, H3, and L3. (Right) The electrostatic potential surface is shown in the same views, with the V2c peptide in sticks representation. (B) Detailed interactions between NCI05 and the V2c peptide. The peptide and NCI05 paratope residues are shown in sticks representation, with other regions of NCI05 shown as cartoon. Hydrogen bonds and salt bridges between the interacting atoms are indicated with gray dashed lines. (C) Sequence and paratope of NCI05. Heavy- and light-chain paratope residues are highlighted in green. NCI05 residues were numbered according to Kabat nomenclature.