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. 2002 Mar 15;30(6):1325–1332. doi: 10.1093/nar/30.6.1325

Figure 1.

Figure 1

Chemical modification of murine CAD by TNBS and NAI. Free GST-mCAD was modified by TNBS (top) and NAI (bottom) for the indicated time (1.5, 3, 6, 7.5 and 9 min). Subsequently the residual nucleolytic activities of the aliquots withdrawn from the modification reaction mixtures at the indicated time points were measured by a plasmid DNA cleavage assay (10 min). TNBS and NAI inactivate free GST-mCAD in a time- and dose-dependent manner. (ST, length standard; S, substrate DNA; C, control reaction; oc, open circular DNA; lin, linear DNA; sc, supercoiled DNA.)