Skip to main content
. 2002 Mar 15;30(6):1325–1332. doi: 10.1093/nar/30.6.1325

Figure 4.

Figure 4

DNA binding by the GST-mCAD variants. DNA binding of the GST-mCAD variants was investigated using a DNA–cellulose binding assay (16). Caspase-3-treated GST-CAD/hICAD-L complex (2.5 µg) and GST (2.5 µg) were incubated with 50 µl of a DNA–cellulose suspension. After washing, bound proteins were eluted by incubating the suspension for 5 min at 95°C in 10 µl SDS-gel loading buffer and subsequently analysed by SDS–PAGE. As can be seen, all GST-mCAD variants bind to DNA–cellulose, whereas GST alone does not.