Figure 5.

Biofilms of PAO1 treated with ZnPor or PEV2 alone, and in combination in the absence of photoactivation. Biofilms of strain PAO1 were grown on coupons cut from polyethylene in a CDC-approved bioreactor. The biofilms were then exposed to (A) PBS (control) or PEV2 (MOI 10:1), or ZnPor and ZnPor/PEV2 exposure at ZnPor concentrations of (B) 6.5 µg/mL, (C) 13 µg/mL, or (D) 33 µg/mL. For joint ZnPor/PEV2 experiments, biofilms were pre-treated with ZnPor for 2 h followed by the addition of phage PEV2 (MOI 10:1) and incubated for 4 h. Biofilms were stained with LIVE/DEAD ^TM fluorescent stain and imaged using confocal laser scanning electron microscopy (CLSM) at 60×. Green = LIVE, red = DEAD. Control refers to a biofilm that received no treatment.