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. 2023 Apr 3;12(4):867. doi: 10.3390/antiox12040867

Figure 6.

Figure 6

Effect of the Eucalyptus EOs on oxidative stress. J774A.1 macrophages pretreated with the EOs before being stimulated with LPS and INFγ. Representative histograms and relative quantification of DCF-DA fluorescence in J774A.1 cells (A,B). Relative mRNA levels of gclc, gclm and hmox-1 in J774A.1 macrophages determined by RT-PCR analysis after 6 h (C). Values are expressed as mean ± SEM from three independent experiments. °° p < 0.01 indicates significant effect of LPS/INF-γ-stimulated cells (STIM) compared to unstimulated cells (CTRL); ** p < 0.01, *** p < 0.001, **** p < 0.0001 indicate significant effect of Eucalyptus extracts compared to stimulated cells. EA = E. alba, EG = E. eugenioides, EF = E. fasciculosa, ER = E. robusta and ES = E. stoatei.