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. 2023 Apr 18;12(4):773. doi: 10.3390/antibiotics12040773

Figure 4.

Figure 4

Click reaction between azide-peptide, K(N3)-RLLR (tR: 5.3 min) and the alkynyl-resorcinarene CTpH(F), (rctt, tR: 9.6 min). (a) Reaction monitoring by means of RP-HPLC at time 0 and 1.5 h. (b) Chromatographic profile, and MALDI-TOF MS spectrum of purified conjugate: CTpH(F)-(AAC-(K)-RLLR)4 (rctt). (c) CTpH(F)-(AAC-(K)-RLLR)4 (rctt) HR-MS, the signals corresponding to isotopic distribution of [M+4H]4+ species are showed; monoisotopic experimental M is 3846.28 uma.