Table 1.
Peptide, alkynyl-resorcinarenes precursors, and synthesized conjugates. RP-HPLC and MS characterization summary.
| Peptides a | RP-HPLC | MS | |||
|---|---|---|---|---|---|
| tR
(min) |
Purity b (%) |
Theorical c [M+H]+ | m/z [M+H]+ | ||
| RLLR | 2.4 | 97.2 | 556.0 | 556.2 | |
| RRWQWR | 4.1 | 92.5 | 986.5 | 986.5 | |
| K(N3)-RLLR | 5.3 | 98.2 | 710.5 | 709.9 | |
| K(N3)-RRWQWR | 5.4 | 83.7 | 1139.6 | 1139.4 | |
| Alkynyl-resorcinarene | RP-HPLC | Yield (%) | MS | ||
| tR (min) |
Purity b
(%) |
Theorical c [M+H]+ | m/z [M+H]+ | ||
| CTpH(F) (rctt) | 9.6 | 97.5 | 58.9 | 1009.3 | 1009.3 |
| CTpH(F) (rccc) | 9.8 | 77.9 | 20.8 | 1009.3 | 1008.7 |
| (Peptide)4-resorcinarene conjugates | RP-HPLC | Yield (%) | MS | ||
| tR (min) |
Purity b
(%) |
Theorical c [M+H]+ | m/z [M+H]+ | ||
| CTpH(F)-(AAC-(K)-RLLR)4 (rctt) | 6.0 | 96.4 | 75.6 | 3847.2 | 3847.5 |
| CTpH(F)-(AAC-(K)-RLLR)4 (rccc) | 6.0 | 88.7 | 60.1 | 3847.2 | 3844.2 |
| CTpH(F)-(AAC-(K)-RRWQWR)4 (rctt) | 6.4 | 74.6 | 56.6 | 5570.4 | 5575.2 |
a All peptides have amide group at C-terminal end; b Chromatographic purity; c M: monoisotopic mass.