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. 2002 Mar 15;30(6):1338–1345. doi: 10.1093/nar/30.6.1338

Figure 3.

Figure 3

Uptake of HK and HHK4b-associated complexes in BAE and MDA-MB-231 cells. The fluoresceinylated polymers (7.5 nmol HK or 0.375 nmol HHK4b) were initially incubated with plasmid DNA (0.75 µg) for 30 min in OptiM, and cationic liposomes (1.5 µg) were then added to form the ternary complex. BAE and MDA-MB-231 cells were incubated with the complexes for 30 min as described in the Materials and Methods. Cell fluorescent density was recorded at 520 nm by using the fluorescent multi-well reader at an excitation wavelength of 485 nm. Data represent the mean ± SEM of replicate experiments.