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. 2023 Apr 19;12(4):960. doi: 10.3390/antiox12040960

Figure 1.

Figure 1

Colchicine inhibited endothelial senescence and restored the relative protein expression of aging-associated biomarkers. (A) Senescence in endothelial cells after 24 h of treatment with different conditions. (B) Colchicine subdued ethanol (EtOH)-induced senescence in endothelial cells. (C) Western blot showing protein expression of P21, KU80, and KU70. (D) Colchicine attenuated the relative protein expression of P21 in endothelial cells after ethanol exposure. (E,F) Colchicine recovered the relative protein expression of KU70 and KU80 in endothelial cells treated with ethanol. α-Tubulin was used as a loading control. Data are the mean of independent biological triplicates. The data was analyzed by performing one-way ANOVA followed by Tukey’s test. Scale bar = 100 µm; error bars represent the SD (**** p < 0.0001, ** p < 0.01, and * p < 0.05).