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. 2023 Apr 19;12(4):960. doi: 10.3390/antiox12040960

Figure 6.

Figure 6

Colchicine abated the relative mRNA and relative protein expression of matrix metalloproteinase (MMP)2. Colchicine did not ameliorate the relative mRNA expression of (A) MMP1 or (D) MMP-11. Ethanol and colchicine did not alter the relative mRNA expression of (C) MMP-10 or (F) tissue inhibitor of metalloproteinase (TIMP)2. Colchicine attenuated relative mRNA expression of (E) TIMP1 and (B) MMP-2 and relative protein expression of (G,H) MMP-2 in ethanol-treated cells. β-actin for qPCR and α-tubulin for WB were used as a loading control. qPCR data are the mean of three independent technical replicates and WB data are the mean of the independent biological triplicates. The data was analyzed by performing one-way ANOVA followed by Tukey’s test. Error bars represent the SD (**** p < 0.0001, *** p < 0.001, ** p < 0.01, and * p < 0.05), ns: not significant.