Table 3.
Gene expression of key cardiac ion channels and receptors in cells cultured on fibronectin (FM) and Cellvo™ Maturity Matrix (MM). The data are expressed as mean ± SEM transcripts per million (TPM).
| Gene | Protein | FM (TPM) | MM (TPM) |
|---|---|---|---|
| ADRB1 | β1 adrenergic receptor | 28 ± 10.7 | 11 ± 2.4 |
| ATP1A1 | Na+/K+ ATPase | 13,449 ± 1132.2 | 16,443 ± 1493.5 |
| CACNA1C | Voltage-gated Ca2+-channel—Cav1.2 | 4344 ± 265.8 | 6229 ± 374.5 |
| CHRM1 | Muscarinic acetylcholine receptor M1 | 1 ± 0.3 | 0 ± 0.2 |
| CHRM2 | Muscarinic acetylcholine receptor M2 | 1167 ± 133.4 | 2757 ± 430.8 |
| HCN2 | hyperpolarization-activated cyclic nucleotide-gated channel 2 | 379 ± 19.6 | 302 ± 15.2 |
| HCN4 | hyperpolarization-activated cyclic nucleotide-gated channel 4 | 1721 ± 237.7 | 2352 ± 463.7 |
| KCNA5 | Voltage-gated K+-channel—Kv1.5 | 16 ± 8.3 | 9 ± 4.1 |
| KCNE1 | Kv-channels β subunit—minK | 81 ± 5.6 | 84 ± 8.4 |
| KCNH2 | Human Ether-à-go-go-Related Gene—hERG | 2811 ± 133.4 | 2411 ± 118.3 |
| KCNJ11 | ATP-sensitive K+ channel—Kir6.2 | 113 ± 7.7 | 148 ± 24.2 |
| KCNJ2 | Inward-rectifier K+ channel—Kir2.1 | 43 ± 2.4 | 35 ± 0.6 |
| KCNJ4 | Inward-rectifier K+-channel—Kir2.3 | 147 ± 11 | 149 ± 17.3 |
| KCNQ1 | Voltage-gated K+-channel—Kv7.1 | 1006 ± 71.6 | 925 ± 47 |
| MYH6 | Myosin heavy chain 6 | 20,943 ± 1256.1 | 37,919 ± 1177.9 |
| MYH7 | Myosin heavy chain 7 | 218,434 ± 11,851.5 | 212,866 ± 9265.9 |
| MYL2 | Myosin light chain 2 | 67,277.5 ± 2061.8 | 43,888 ± 1100.6 |
| MYL4 | Myosin light chain 4 | 55,748 ± 3432.8 | 45,843 ± 1119.1 |
| PDE3A | Phosphodiesterase 3A | 62 ± 6.8 | 51.5 ± 42.9 |
| SCN1B | Voltage-gated Na+-channel β1 subunit | 47 ± 4.3 | 31.5 ± 2 |
| SCN5A | Voltage-gated Na+-channel—NaV1.5 | 8197 ± 406.9 | 8277.5 ± 618.3 |
| TNNC1 | Troponin I | 60,057.5 ± 2139.7 | 45,849.5 ± 1828.3 |