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. 2023 Mar 27;10(4):423. doi: 10.3390/bioengineering10040423

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The fallopian tube explants were cultured in a microfluidic platform for sEV collection. (A) The workflow of hFTE-derived EV proteomics characterization. (B) The live/dead staining of hFTE epithelium cultured in the PREDICT-MOS for six days. The green fluorescence (calcein) indicates live cells, and the red fluorescence indicates dead cells, labeled by BOBO-3 iodide. Immunohistochemistry staining of FOXJ1 and PAX8 confirmed that the hFTE maintained the two major epithelial cell populations, ciliated and secretory cells, after six-day dynamic culture on the PREDICT-MOS. Scale bar = 10 µm.