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. 2023 Apr 8;13(4):660. doi: 10.3390/biom13040660

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of BTK and ITK phosphorylation on Cx43 regulation in the HEK-293T cells as observed through changes in detergent solubility. Cx43 was extracted with 1% Triton X-100 from HEK-293T parental (HEK^Par) and (A) stably expressing BTK (HEK^BTK) or (B) stably expressing ITK (HEK^ITK) cells. Equal amounts of the total protein fraction (T), the Triton X-100 soluble fraction (S), and the insoluble fraction (I) were subjected to SDS-PAGE and blotted with an antibody against Cx43. Bands were quantified using ImageJ (all units measured in pixels) and GraphPad Prism. Statistical data represent one-way ANOVA (n = 3, ** p < 0.01).