Figure 6.

The effect of BTK and ITK phosphorylation on the Cx43 gap junction intercellular communication in HEK-293T cells. A scrape loading dye transfer assay was used to assess communication from HEK-293T parental (HEK^Par) and (A) stably expressing BTK (HEK^BTK) or (B) stably expressing ITK (HEK^ITK) cells. Representative confocal images (Green, Lucifer yellow) are provided. Statistical data were calculated by ImageJ (all units measured in pixels) and GraphPad Prism by measuring the surface area of the transferred dye compared to HEK^Par. Data represent the Student’s t-test (n = 3, ** p < 0.01; **** p < 0.0001).