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. 2023 Apr 8;13(4):660. doi: 10.3390/biom13040660

Figure 7.

Figure 7

The effect of BTK and ITK phosphorylation on Cx43 regulation in lymphocytes as observed through changes in electrophoretic mobility. Western blot of (A) Daudi cells with and without BTK activation using the α-IgM antibody or (B) Jurkat cells with and without ITK activation using α-CD3/α-CD28 antibodies. Antibodies used were labeled on the right of each blot. Arrows point to the different migrating Cx43 bands from the electrophoresis. Data were quantified using ImageJ (all units measured in pixels) and GraphPad Prism by Student’s t-test (n = 3, ** p < 0.01; **** p < 0.0001).