Skip to main content
. 2023 Apr 3;12(4):870. doi: 10.3390/antiox12040870

Figure 4.

Figure 4

Sal A-mediated protection against APAP involves SIRT1 activation. (A) Sal A pretreatment was performed for 3 days followed by APAP stimulation in mice. SIRT1 protein expression in the liver, n = 3. (B) AML12 cells were incubated with Sal A before exposure to APAP. SIRT1 protein expression in AML12 cells, n = 3. (C) AML12 cells were transfected with control siRNA or SIRT1 siRNA for 24 h and then incubated with Sal A (10 μM) for 6 h. SIRT1 expression in AML12 cells, n = 3. (D) AML12 cells were transfected with SIRT1 siRNA or negative control and then incubated with Sal A and APAP. SIRT1, p66Shc, MnSOD and NF-κB protein expressions in AML12 cells, n = 3. (E) Immunofluorescence analysis of SIRT1 (100× magnification). * p < 0.05, ** p < 0.01 vs. control or si-control. # p < 0.05, ## p < 0.01 vs. APAP or si-con+Sal A. & p < 0.05, && p < 0.01 vs. APAP+Sal A.