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. 2023 Apr 3;12(4):870. doi: 10.3390/antiox12040870

Figure 5.

Figure 5

miR-485-3p regulates SIRT1 expression in AML12 cells. (A) Sal A pretreatment was performed for 3 days followed by APAP stimulation in mice. miR-485-3p expression in mice, n = 3. (B) AML12 cells were incubated with Sal A before exposure to APAP. miR-485-3p expression in AML12 cells, n = 3. (C–F) Ago-485-3p, ant-485-3p or negative control was transfected into AML12 cells. (C,E) miR-485-3p expression, n = 3. (D,F) SIRT1 expression, n = 3. (G) Dual luciferase assay. Luciferase constructs containing wild-type or mutant SIRT1-3′-UTR and ago-485-3p or negative control were cotransfected into AML12 cells followed by Sal A treatment, n = 3. (H) Luciferase constructs with 3′UTR-wt or 3′UTR-mut of SIRT1. BS, binding site. * p < 0.05, ** p < 0.01 vs. control, ago-NC or ant-NC. # p < 0.05, ## p < 0.01 vs. APAP.