Figure 3.

Induction of m5α-R2 gene expression in adult female mice brains by the administration of progesterone. m5α-R2 gene expression was detected by in situ hybridization (A), and semi-quantitative RT–PCR (B) in the brains of female adult mice to which 500 µg of progesterone had been administrated 4 h previously. (A) m5α-R2 gene expression induced by progesterone was detected in the hippocampus. (B) Bands were obtained by semi-quantitative RT–PCR on total RNA extracted from individual mouse brains with or without the administration of progesterone. HDC was used as a positive control for progesterone administration (37). m5α-R2 gene expression was induced by progesterone in the female adult brain.