Figure 3.

Effect of Lactobacillus spp. on gut microbiota composition at the phylum level and diversity and taxonomic profiling in C57BL/6J mice. C57BL/6J mice (n = 6/group) were grouped into the NC and Amp groups. The groups given the three-strain combination were fed a dose of 1.6 × 10⁸ CFU/mouse (LABL) or 4.8 × 10⁸ CFU/mouse (LABH) from the 3rd day until the 7th day. All groups were analyzed for 16S ribosomal RNA V3–V4 gene expression in the ileum on the 12th day. (a) Phylogenetic cladogram from the LEfSe analysis, (b) Firmicutes spp., (c) Verruocrobia spp., (d) Actinobacteria spp., (e) Bacteroidetes spp. (f) B/F ratio communities in the control and Amp groups. (g) α-diversity of intestinal microbial communities. (h) β-diversity of intestinal microbial communities. *, compared with NC group, p < 0.05; #, compared with Amp group, p < 0.05, Ψ, compared with LABL group, p < 0.05.