Table 2.
List of primers used to analyze mice intestinal gene sequences used in RT-PCR analysis.
| Gene | Primer Sequence (5′–3′) * | Size (bp) | GenBank Accession No. |
|---|---|---|---|
| GADPH (mice) | F-GCACAGTCAAGGCCGAGAAT | 151 | JN958248.1 |
| R-GAATCCTTCTGACCCATGCC | |||
| Claudin2 | F-TCCGGGACTTCTACTCACCA | 190 | XM_021188667.2 |
| R-CTCCTAGTGGCAAGAGGCTG | |||
| Claudin3 | F-GTTTCGGCATTCATCGGCA | 180 | XM_021186977.2 |
| R-TGCCAGTAGGATAGACACCAC | |||
| Claudin5 | F-GCTCTCAGAGTCCGTTGACC | 235 | NM_013805.4 |
| R-CTGCCCTTTCAGGTTAGCAG | |||
| IL-6 | F-TCTCTCCGCAAGAGACTTCCA | 235 | XM_021191538.1 |
| R-ATACTGGTCTGTTGTGGGTGG | |||
| TNFα | F-CTAGCCAGGAGGGAGAACAG | 149 | NM_001278601.1 |
| R-GCTTTCTGTGCTCATGGTGT | |||
| IFNγ | F-TCCTTTGGACCCTCTGACTT | 130 | NM_008337.4 |
| R-GTAACAGCCAGAAACAGCCA | |||
| Tph1 | F-ACTGCGACATCAGCCGAGAA | 162 | XM_036152912.1 |
| R-CGCAGAAGTCCAGGTCAGAAATC | |||
| Mao | F-GGAGAAGCCCAGTATCACAGG | 113 | NM_173740.3 |
| R-GAACCAAGACATTAATTTTGTATTCTGAC | |||
| VEGF-α | F-GCT ACT GCC GTC CGA TTG A | 163 | NM_001025257.3 |
| R-ATG GTG ATG TTG CTC TCT GA | |||
| Pten | F-GGA AGT AAG GAC CAG AGA CAA | 287 | XM_006526769.3 |
| R-CAC CAC ACA CAG GCA ATG |
* F: Forward primer; R: reverse primer.