Table 1.
Data was collected from the PRF process using the rat model in the last five years, including one from 2023.
| Author and Year | Breed | Animal Size | Number of Animals | Blood Collected (mL) |
Centrifuge (RPM/min or RCF) |
Donor Area |
|---|---|---|---|---|---|---|
| Akyildiz et al. (2018) [44] | Sprague-Dawley rats | 340–380 g | 23 | 4 | 3000 rpm/10 min | Intracardiac puncture |
| Alizadeh et al. (2023) [45] | Wistar rats | 230–300 g | NR | NR | 2700 rpm/12 min | Orbital sinus |
| Alsherif et al. (2020) [46] | Wistar rats | 300–360 g | 10 (PRF group) |
4 | 2000 rpm/10 min (400 RCF) | Orbital sinus |
| Awadeen et al. (2020) [47] | Sprague-Dawley rats | NR | 63 | 10 | 3000 rpm/10 min | Orbital sinus |
| da Silva et al. (2022) [11] | Wistar rats | 350–450 g | 24 | 3 | 2700 rpm/12 min (701 RCF-max) and 1500 rpm/14 min (216 RCF-max) | Intracardiac puncture |
| Demirel et al. (2018) [48] | Sprague-Dawley rats | 400–450 g | 28 | 16 | 3000 rpm/13 min | Intracardiac puncture |
| Engler-Pinto et al. (2019) [42] | Wistar rats | 250–300 g | 8 | 3.5 | 2700 rpm/12 min | Intracardiac puncture |
| Grecu et al. (2019) [49] | Wistar rats | 220–420 g | 35 | 10 | 1300 rpm/8 min (400 RCF) | Intracardiac puncture |
| Grecu et al. (2019) [50] | Wistar rats | 220–420 g | 35 (scarified to produce PRF) | 10 | 1300 rpm/8 min (400 RCF) | Intracardiac puncture |
| Huang et al. (2020) [23] | Sprague-Dawley rats | 180–220 g | 24 (sacrificed to produce PRF) | 5 | 400 g/10 min | NR |
| Jamalpour et al. (2022) [51] | Wistar rats | 300–350 g | 60 | 2 | 1500 rpm/14 min and 2700 rpm/12 min | Orbital sinus |
| Mirhaj et al. (2022) [52] | Wistar rats | 250–300 g | 3 (PRF group) |
NR | 2700 rpm/12 min | Orbital sinus |
| Mourad et al. (2022) [53] | Wistar rats | 250–300 g | 30 | 2 | 3000 rpm/10 min | Tail vein |
| Neves-Atti et al. (2022) [54] | Spontaneously hypertensive rats | 250 g | 40 | 6 | 3000 rpm/10 min | Intracardiac puncture |
| Nica et al. (2019) [55] | Wistar rats | 460–550 g | 40 | 9 to 12 | 450 g/12 min | Intracardiac puncture |
| Nugraha et al. (2018) [56] | Wistar rats | 250 g | 36 | 1.5 | 3000 rpm/10 min | Intracardiac puncture |
| Özçay et al. (2018) [22] | Sprague-Dawley rats | 250–300 g | 40 | 1 | 3000 rpm/10 min | Intracardiac puncture |
| Özçay et al. (2020) [40] | Sprague-Dawley rats | 250–300 g | 10 (PRF group) | 1 | 3000 rpm/10 min | Intracardiac puncture |
| Padilha et al. (2019) [57] | Wistar rats | 300–400 g | 1 animal scarified per group | 9 | 3000 rpm/12 min | Intracardiac puncture |
| Rady et al. (2022) [19] | Wistar rats | 175–200 g | 36 | 2 | 3000 rpm/10 min | Tail vein |
| Silveira et al. (2022) [58] | Wistar rats | 320 g | 54 (2 animals scarified for PRF) | 10 | 2700 rpm/12 min (857 RCF max) | Intracardiac puncture |
| Sumida et al. (2019) [59] | Wistar rats | 400–450 g | 23 | 6 | 890 g/13 min | Intracardiac puncture |
| Tavakoli et al. (2022) [60] | Wistar rats | 400 g | NR | 6 | 1500 rpm/14 min | Orbital sinus |
| Tayşi et al. (2018) [61] | Sprague-Dawley rats | 240–260 g | 60 (including a sacrigication group) | 10 to 15 | 3000 rpm/10 min (400 RCF) | Intracardiac puncture |
| Torul et al. (2018) [39] | Wistar rats | 200–250 g | 30 | 2 | 3000 rpm/10 min (400 RCF) | Tail vein |
| Vares et al. (2021) [62] | Wistar rats | 250–280 g | 32 | 2 | 3000 rpm/12 min (400 RCF) | Intracardiac puncture |
| Wang et al. (2021) [41] | Sprague-Dawley rats | NR | 30 | 5 | 400 RCF/10 min | Abdominal aortic |
| Zhang et al. (2019) [63] | Sprague-Dawley rats | 210–310 g | 20 | 5 | 3000 rpm/10 min | Abdominal aortic |
| Zhang et al. (2022) [64] | Sprague-Dawley rats | 250–300 g | 32 | 3.5 | 600 RCF | Intracardiac puncture |
RCF = relative centrifugal force; NR = not reported.