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. 2023 Apr 18;12(4):952. doi: 10.3390/antiox12040952

Figure 4.

Figure 4

Semi-quantitative analyses of protein or mRNA levels of CLD-2, OCLN, and ZO-1 (TJP1) after 100 µM flavonoid exposure for 48 h in MDCK II cells. (A) Western blotting analysis of CLD-2 expression in cell lysates from the control (DMSO) and 100 µM flavonoid-treated MDCK II cells. (B) Quantitative analysis with densitometry of 100 µM flavonoid-treated MDCK II cells. HST, QUE: n = 8. (C) RT-qPCR analysis of CLD-2, OCLN, and TJP1 expressions in cell lysates from the control (DMSO) and 100 µM flavonoid-treated MDCK II cells. Error bars indicate standard deviations. Statistical analyses were performed using the Turkey–Kramer multiple comparison tests. Difference from the value of the control cells, * p < 0.05. HST, QUE: n = 3.