Figure 6.

Effect of MDA-1833 secretome on osteoclast/osteoblast co-cultures. (a) Timeline of the resorption assay in a co-culture setting. Mature osteoclasts were seeded with osteoblasts and exposed to MDA-1833-conditioned media for three days. (b) Representative micrographs of the surface of bone slices after three days of resorption in co-cultures. Resorption events are highlighted by black arrows. Scale bar—200 μM. (c) Estimated percentage of eroded surface, (d) trench percentage per total eroded surface and (e) TRAcP activity quantified at the end of the co-culture experiment. Data are expressed as the median of individual data points from six independent experiments (Friedman’s test).