Figure 4.

Overexpression of Mfn2 attenuated isoprenaline-induced cardiomyocyte injury by promoting mitochondrial fusion. Adenoviruses Ad-Mfn2 and Ad-control were used to transfect cardiomyocytes and then were stimulated with Iso (10 µM) for 48 h. (A,B) Western blot showing the expression of PINK1 over time. (C) Mitochondrial morphology in cardiomyocytes was shown by TEM. Details of mitochondrial structures are shown in the red frame. (D–F) Representative immunofluorescence images of mitochondrial morphology. Mitochondria are shown in green, and nuclei are shown in blue. (G,H) Fluorescence images of MMP was detected by JC-1 tracker. J-aggregate staining is shown in red and J-monomer staining is shown in green. (I) Mitochondrial respiration was measured using extracellular oxygen consumption assay kits to assess oxygen consumption rate. Statistical analyses (A–I) were performed by one-way ANOVAs followed by Dunnett’s test post-hoc tests. Data are presented as the mean ± SD. (n = 3). * p < 0.05.