Skip to main content
. 2023 Mar 24;13(4):589. doi: 10.3390/biom13040589

Figure 5.

Figure 5

Design of CRISPR target in A. domesticus. (a) Schematic of the annotated Ad vermilion gene from A. domesticus genome data. Red box: ATG starting code; Yellow boxes: exons; Purple arrow: sgRNA site. Note: in this figure intron sizes are not proportional to exons; (b) Ad muscle actin gene with promoter areas. Red box: Sequences used for promoter prediction; Green sequences: predicted promoters; Red sequences: start codon; Yellow sequences: protein coding area; Gary blue area: promoter sequence used in CRISPR knock-in construct.