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Φ29 TP–DNA replication by wild-type and mutant Φ29 DNA polymerases. Autoradiograph of Φ29 TP–DNA replication assay carried out as described in Materials and Methods in the presence of 5 ng wild-type (wt) or mutant Φ29 DNA polymerase. After incubation for 10 min at 30°C, relative activity values were calculated (see Table 1) and the length of the synthesized DNA was analyzed by alkaline agarose gel electrophoresis. The migration position of unit length Φ29 DNA is indicated.
