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. 2002 Mar 15;30(6):1379–1386. doi: 10.1093/nar/30.6.1379

Figure 3.

Figure 3

Φ29 DNA polymerase mutants are strongly affected in Φ29 TP–DNA amplification. The assay was carried out as described in Materials and Methods, in the presence of 5 ng wild-type or mutant Φ29 DNA polymerase, 5 ng Φ29 TP and 10 µg each Φ29 DBP and Φ29 SSB. After incubation for 90 min at 30°C, samples were processed and the amplified DNA was analyzed by alkaline agarose gel electrophoresis as described in Materials and Methods. (Top) Autoradiograph of such a gel; (bottom) Φ29 TP–DNA amplification signal detected by ethidium bromide staining. The migration position of unit length Φ29 TP–DNA (19 285 bases) is indicated.