Figure 6.

Interaction between DNA polymerase and TP shown by glycerol gradient centrifugation. Wild-type or mutant DNA polymerase (8 µg) was incubated with TP (4 µg). After incubation for 30 min at 4°C, samples were loaded on top of a continuous 15–30% glycerol gradient (4 ml) and centrifuged as described in Materials and Methods. Gradients were fractionated and subjected to 12% SDS–PAGE. Gels were stained after electrophoresis with Coomassie Blue. Densitometric quantitations in arbitrary units of both Φ29 DNA polymerase (full circles) and Φ29 TP (open circles) are represented.